Oliver Twist Essay

In ‘Oliver Twist’ Bill Sykes brings terror to the novel, which adds to the suspense and makes you want to read on. Bill Sykes is brutal which you soon realise when he delights in beating his dog or girlfriend, Nancy. Many novels of this time were full of such characters and Dickens, a playwright and an actor as well as a novelist, knew then well. The orphan Oliver Twist escapes from the workhouse by running away to London. Oliver is introduced to Fagin who trains him to be a thief and he is then taken on a burglary with Sykes, Oliver recovers from being shot in the burglary and Nancy nurses Sykes back to full health. Sykes kills Nancy, which has a terrible effect on him, and he later kills himself. Bill Sykes brings the real terror to the novel but he is also extremely unpredictable. Rarely he can seem quite reasonable and occasionally shows kindness to his dog and girlfriends, but generally he keeps everyone in fear of what he is going to do next. We see evidence of this when Fagin says, â€Å"You won’t be too violent Bill? † This statement shows that they have no trust in him. Bill Sykes also has a problem with alcohol, he drinks far too much, and this probably accounts for some of his unpredictable behaviour. We see evidence of his drinking problem when he says, â€Å"†¦ to prevent my standing treat for a pint of so†¦. † This shows that he thinks that he thinks drinking is a treat and does not himself think that he has a drinking problem. Bill Sykes us the archetypal Victorian villain. To be a real villain Sykes must show no compassion for anyone and we see that on many occasions. When Nancy has nursed him back to health, staying up for nights looking after him, he turns on her the moment he wakes up. This shows he is very ungrateful for any sympathy given to him. â€Å"Illness had not improved Mr Sykes’ temper, for as the girl raised him up and led him to a chair, he struck her †¦. † He is also quite ready to send Nancy out to work and possibly profit from what she does. From all of this we can gather further evidence that he is ungrateful for any help he received. Sykes treats his dog very badly, it receives regular kicks and is threatened constantly. At one time when he was going to escape he contemplates killing, â€Å"The dog, though. If any descriptions of him were out, it would not be forgotten that the dog was missing. † Sykes is a typical bully who shows no remorse for what he does to others. We see evidence of this when he takes Oliver on the burglary with him and does not care whether Oliver gets injured. Sykes has no respect for Fagin and you can tell this by the way he speaks to him. You can see Fagin is afraid of him but Fagin does like to have the last word. If Sykes does not get his own way he will turn to violence as we see with the arguments over Oliver, â€Å"†¦ looking sternly at him, and ostentatiously passing a pistol into a more convenient pocket. â€Å"That’s lucky for one of us. † This shows again Sykes is unpredictable and a bully and brings more tension. When Bill Sykes sees Fagin, Fagin changes the tone of his voice at once. Fagin is obviously afraid of the physical strength of Sykes but Sykes is still wary of Fagin.

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Stricter Guidelines for Concussion Testing in High School Athletics Research Paper

Stricter Guidelines for Concussion Testing in High School Athletics - Research Paper Example Concussion results from a blow to the skull and instigates a disorder of working of the nerve cells in the brain. This means that division of the functions of the brain are for the time being 'on hold'. The indications comprise impermanent oblivion,  headache  and, most of the times, a memory loss about the dangerous incident. Nausea and vomiting are also general symptoms. As stated by Michael p. MacClenny â€Å" American athletes suffer 300 000 concussive injuries on a yearly basis and 19% of participants in contact sports such as football and rugby are likely to suffer a mild traumatic brain injury (TBI) during a season†. The rate of recurrence of this injury, with a lack in understanding of scientific healing methods and physiology, has been the reason to augmented consideration from the world of sports medicine. The generally hotly discussed concern in sports concussion circles around efforts to set up a scientifically based guide for going back-to-play restrictions, g iven that a subsequent effect prior to previous one from a first concussion can have harmful effects. According to Hovda DA â€Å"This idea is based on recent animal models which suggest that symptoms of sports concussion are likely related to acute metabolic dysfunction† Post -traumatic hectic glycolysis and simultaneous reduced flow of cerebral blood have been concerned for the reason of this dysfunction. It is assumed that metabolic disorder, unless fully treated, may intensify the neurological susceptibility of the athlete if a following shock (even slight one) is continued. The second controversial influence disorder and less stern, although potentially debilitating post-concussion patterns are two dangers concerned with returning a sportsperson to engage in a physical activity before absolute recovery. According to Collins MW â€Å"Although long-term deficits in the form of post-concussion syndrome have been observed from a single concussive event , it is typically ass umed that proper management of injury should lead to a good prognosis and minimal, if any, long-term neurological deficits.† As a result, the exact supervision of concussion is necessary in protecting athletes from an enduring cognitive injury or else death. The great amount of concussive wounds are experienced at the high school and sophomoric levels of involvement, nonetheless many trainers, athletic coaches and team doctors may possibly be ignorant of the harsh penalties and consequences that can take place in returning an athlete to participate ahead of time. As stated by Taylor and Francis â€Å"Recent studies claim that the average concussion ameliorates within 1 week of onset and many concussion grading scales also clear mildly and moderately injured athletes for participation within 1 week.† It is the hypothesis of the author that an even slight concussion frequently needs more than seven days to entirely treat. Concussions have forever been a component of physi cal sports; however with players turning into stronger and bigger, some steps have to be done to increase consciousness of the seriousness of concussions and what may occur afterward down the road if sportsperson are not allocated the sufficient quantity of time to get well. According to Lindsey Newman â€Å" The National Football League has already put regulations on how long a player has to stay out after

America Online and Time Warner Case Study Example | Topics and Well Written Essays - 500 words

America Online and Time Warner - Case Study Example Since then, this merger has been regarded as one of the biggest corporate mismanagement examples in the realm of managing telecommunication network and media companies. The case study unveils a number of issues that are big threats to the telecom sector and media companies globally. In 2010, media analyst Tim Arango of the New York Times attempted to throw some light on the merger between AOL and Time Warner that took place a decade ago from then. In analyzing the reason behind the merger, Arango remarks that at the wake of the 21st century, experts in telecomm and media industries were expected Internet was going to become the main medium of content delivery and entertainment all over the world, replacing and/or amalgamating the various traditional media and content delivery systems of that time. However, this was the main issue with the decision makers at both AOL and Time Warner. AOL’s business outreach and technological equipment and expertise were not as robust as to handle the huge industrial momentum of Time Warner. Probably, AOL managers were thinking well ahead of their time. A scrutiny of scientific literature suggests that Internet technologies were not as advanced at that time as to facilitate all the services needed for international distrib ution of various types of contents. Even in the middle of the first decade of the new millennium, scientists were striving to establish a sufficiently powerful architecture for the purpose of â€Å"pervasive content delivery† (Su and Chi, 82). Clearly, Internet technologies had limited strength during the merger between AOL and Time Warner. The case study thus helps us to understand that we need a really powerful and futuristic computing system to ensure Internet based content delivery of all kinds of information ranging from news to cinemas, weather forecasting to cartoons, and so on. As shown by the case study discussed, technological barriers remain a prominent issue

Effective Leadership Style in Controlling the High Employee Turnover Thesis

Effective Leadership Style in Controlling the High Employee Turnover Rate within Nursing Faculties - Thesis Example The nursing facilities in UK are experiencing a 12.2% reduction as of 2010 (Buchan and Seccombe, 2012, p. 21). In Canada, â€Å"a shortage of 20,000 nurses† was reported in 2012 (Coutts, Nursing faculty leaders in US, UK, Canada, and Australia are experiencing problems related to recruitment and retention (American Association of Colleges of Nursing, 2013a; National Black Nurses Association, 2013; Laschinger et al., 2009; Longley, Shaw and Dolan, 2007). To solve problems related to recruitment, nursing faculties in US, UK, Canada, and Australia are continuously hiring nurses from different counties around the world (Netzwerk Migration in Europa, 2013). In Canada, low salary is one reason why nursing educators in this area are looking for other more promising job opportunities (Cash, Doyle and Tettenborn, 2011). Nursing faculties in US, UK, Canada, and Australia are having problem retaining nurses in the field of education. Aside from unattractive payment scheme, other reasons that causes poor retention includes lack of trust and collaboration among the nursing staff, over-worked, absence of career opportunities, no proper recognition for good work performance, absence of control over job performance, and poor communication among the faculty members (Hunt, 2009, p. 2). To solve retention, problem, nursing faculties in US, UK, Canada, and Australia will have to use transformational leadership not only to help them create a healthy and attractive work environment which is necessary in increasing the work satisfaction of registered nurses who wish to pursue a career in teaching (Cummings et al., 2009; Nielsen et al., 2009; Raup, 2008). Furthermore, Nielsen et al. (2009) mentioned that there is a strong relationship between the use of transformational leadership style and the decrease in work-related stress and burnout. By solving problems related to stress, burnout and poor working environment, the nursing faculties in US, UK,

Biography of Joaquin Murieta Essay Example | Topics and Well Written Essays - 500 words

Biography of Joaquin Murieta - Essay Example Not much is mentioned about his upbringing. His history shifts to his marriage to Rosa Feliz of Vayoreca. Together with his wife and her three brothers they moved to California for the gold rush. He and his wife settled in Niles Canyon where he toiled as a vaquero and a mustang catcher. After this point the legend of Joaquin Murrieta begins controversy. The first legend depicts Joaquin Murrieta as a bandit while the other one makes him a hero (Ridge, John, 17). In the first legend, Joaquin Murrieta joins his brother-in -laws bandit (Burns, Noble, 11). The bandit kills and steals from white people. After the death of his brother-in-law, Joaquin takes over the bandit’s leadership. He continues to lead the men to crime, and specializes on killing and stealing from the Chinese people. Joaquin and his bandit do not have reservations on killing fellow Hispanics and countrymen. The state of California puts a bounty of 5000 dollars on his head due to the endless menace. After the shooting of General Joshua Bean, Joaquin and Reyes (also his brother-in-law) are implicated. Joaquin fled leaving Reyes to be punished by hanging due to the murder of bean. Overwhelming evidence pointed to Joaquin as the killer (Burns, Noble, 13). Hence a team commandeered by Harry Love was created to hunt him down. Harry Love captured and killed Joaquin. He cut off his head and preserved it in a bottle of alcohol. He later went round the mines to confirm his identity and assure the people that Joaquin would not harm them again. The term bandit is a widely applied in this version of the legend. Joaquin is referred to as a racist, outlaw, murderer, robber and gang leader. Law enforcement and afflicted individuals mainly applied this term. The appropriateness of the term varies depending on the validity of the information. It is hard too tell if the cruelty of Joaquin is exaggerated or not. However, in the context of law enforcers and victims of Joaquin, it is hard to find

Amazon Acquisition and Equity Investment Research Paper

Amazon Acquisition and Equity Investment - Research Paper Example 2- SET OF COMPANIES ACQUIRED BY AMAZON IN 2011 In 2011, set of companies were acquired with price of US $771 million by Amazon (Amazon, 2011). None of the companies are defined specifically but all are aimed at market development for Amazon for increasing customer base as well as sales channel increase in portfolio. Two of the companies included Marshall Cavendish US Children’s Books Titles and LoveFilm International Limited Etc (Amazon, 2013). The change in sales and net income from acquisition was as follows: Set Of Companies in 2011 2011 2010 Sales 48,077 34,204 Change In Sales 40.56% Â   Net income 631 1152 Change In NI -45.23% (Amazon, 2011) The above acquisition also impacted sales positively. But rise in sales is increasingly offset by the downside movement of net income. With acquisition of companies Amazon.com has burdened its net income with additional five percent than magnitude of sales rise. Hence, the acquisition cannot be referred as successful strategy. 3- AQC UISITION OF ZAPPOS.COM, INC. The acquisition was conducted on November 2009 with purchase price of US $.1,134 million (Amazon, 2009). The acquisition was aimed at market penetration and expanding Amazon’s presence in categories shoes and apparel. The impact of Zappos.com Inc on the sales and net income has been highly successful as reflected from the given below change in sales and in net income: ZAPPOS.COM, INC IN 2009 2009 2008 Sales 24,509 19,166 Change In Sales 27.88% Â   Net income 902 645 Change In NI 39.84% (Amazon, 2009) Hence, Zappos.com. Inc acquisition can be regarded as successful from results shown above as not only the sales have increased but the profits have increased as well. ANSWER # 2 The assessment of each of the acquisitions mentioned above with respect to its timing of investment is conducted as follows as discussed in annual reports of the company: FOR Kiva IN 2012 Investment Value $678 million SALES Actual Pro Forma Change 2

Property Law Essay Example | Topics and Well Written Essays - 1250 words - 1

Property Law - Essay Example However, in due course the differentiation between a will and a testament has become thin, and people use the term, will, to refer to a statement that both disposes of both personal property and real property. In the event an individual dies without drafting a will, the state proceeds to distribute the person’s estate in accordance with the laws of Descent and Distribution of the person’s stateii. The significance of a will is diverse from the fact that it gives the drafter a chance to choose the people who will be heirs to his property. It also allows the testator to decide the people who will execute their estate, and using fairness in distributing their wealth, in place of the court appointing a stranger to allocate the estate to the family. Another importance of a will is the fact that the testator can protect the interests of the people close to him, and those of his children in choosing who their guardian would be in the event of the testator’s death. When writing a will the law requires the testator give information as to who will take care of their children incase the stated guard dies before the execution of the will. This may include the other benefactors of the will in the event they die before the execution of the will. ... Requirements for a Will to be Valid The first requirement is that the testator, the person making the will must declare himself as the testator and that he revokes any previous will either express or impliediii. The person should also have the mental capacity to make a valid will at the time of drafting of the will. The person making the will to be accepted by the law as making a valid will through mental capability must be at least 18 years and above, bar for any exceptions provided for by law. Another indication of whether the person has the capacity to make a valid will is that they ought to be of sound mind, understanding, and memory. This is to mean that the person ought to be aware of what they are writing and its implications. It is essential to note that the Mental Capacity Act of 2005 does not have provisions for invalidating a will; that has already been prepared by an individual of unsound mind. There is normally the assumption of intention, in that if a will is validly ex ecuted and the person is of sound mind during the process of execution. This is the third requirement of a valid will; that the testator must have an intention to dispose of their properly as per the will upon the testator’s death. The third requirement is that there should be no traces of undue influence, force and/or fraud. If the court can establish that the testator was either pressured into making the will, or if the execution of the will was through fraudulent means, then it may set aside part of the will, or will in its entiretyiv. It is mandatory for the testator to sign the will or have someone sign it for them; the will must not be necessarily in writing. In most

We live in a patriarchal society discuss how gender links to Essay

We live in a patriarchal society discuss how gender links to inequality in society using examples from the past and present and in England and Arab spring - Essay Example Not only have there been both overt and covert means to ensure that the status quo is maintained, but even in the modern era, it has become common for openly discriminatory actions against women to be promoted at almost every level of society. The world today has seen numerous changes and women have come to experience opportunities that were not open to them for many centuries. It is therefore important that a comprehensive discussion of how gender links to inequality in society be conducted. In Elizabethan England, it was a common belief among individuals in society that women were inferior in all aspects to men. This belief ensured that women were put in a subordinate status where they did not have the same opportunities as those that were given to men (Ellis, 1839). Even Queen Elizabeth I herself seemed to promote this bias and she did this through accepting that she was indeed as physically weak as any woman, but despite this, she was also a woman who had the heart and stomach of a king. It seems that she compared herself thus because she wanted to attribute some of the male features that were believed to be superior to herself. Moreover, even the most prominent writers of the time such as Shakespeare and John Knox, the protestant leader, all believed that women were inferior. The latter especially promoted this belief by stating that women were created in perfection in order to serve and obey man, a statement that shows the predominance of patriarchy in society (Fle tcher, 1994). This attitude towards women continued to be propagated in later centuries and some of its aspects have remained prevalent to this day. The patriarchal nature of English society has led to a situation where women have continued to be discriminated against despite the development of ideals such as gender equality. The sometimes-unconscious discrimination against women in society has led to the continuation of

The differences between islamic banking and conventional Essay

The differences between islamic banking and conventional banking-system - Essay Example s that act as safe deposits of people’s savings without which they will have no place to save and will also face the risk of keeping all money at home. Banks also facilitate lending and people can borrow any amount of money they require in any convenient manner. Banks essentially act as intermediary between depositors who lend money to the band and borrowers to whom the banks lend money. The amount that banks pay to the depositors and the amount that they received from the borrowers are both called interest. Both depositors and borrowers can be individuals, families, organizations, governments and so on. Since at any point of time some depositors withdraw their money, many others do not. This provides the banks opportunity to convert short term deposits, which are their liabilities to long term loans which are their assets. The interests that banks pay to their borrowers are less than the interests that the banks pay to their depositors. This difference serves as income of ban ks all over the world. Although banks play a crucial role in the management of money from depositors and lending money to the needy, banks are also indispensable for national and international payments system. Banks also create money. Individuals, organizations or governments do not only need banks as safe custody of their money, but they all also need to circulate their funds like money getting transferred from â€Å"buyers to sellers or employers to employees or taxpayers to governments† (Gobat). In this case too banks play a prominent role. They handle payments like issuing personal cheques to making electronic payments of large amounts between banks. The payments system is a â€Å"complex network of local, national, and international banks and often involves government central banks and private clearing facilities that match up what banks owe each other† (Gobat). In today’s era of international trade, most payments are administered instantly. An efficiently managed system of payme nts is

Communications & Crisis Essay Example for Free

Communications Crisis Essay The Texas Division of Emergency Management (TDEM) has begun to receive official reports stating that the public water supplies of several towns in the South East Texas (SET) area have become contaminated with life-threatening biological agents. As director of this office, it is my responsibility to address contingency plans within the organization and the public; without creating panic. Areas of focus will include external and internal communication channels, communication advantages and challenges, communication strategies, applicable technology, technology utilization, and media opportunities. Successful management of the situation requires the establishment of teams and communication channels. Organization teams include a state relations team, district relations, media relations, and a crisis communications team (CCT). State, district, and media relations will communicate with CCT acting as coordinator. As the director of TDEM, I will be in charge of the CCT and oversee all organization operations. The state relations team till will be in charge of communications with the Texas Department of Health, the Texas Department of Public Safety, the Texas Emergency Management Council (TEMC), and other government officials. According to Texas law, mayors and county judges serve as emergency management directors, bearing the responsibility for maintaining an emergency management program within their respective jurisdictions (TDEM, 2014). This will help with local emergency coordination’s. The district relations team will engage with local authorities to organize emergency response operations. The media relations team will communicate with local and online media sources and keep them informed of the issue. Our organizations communication channels can instantly connect with multiple organizations at once. This gives our organization a major advantage. We have the ability to spread and collect information quickly and on a grand scale. Information needs to be disseminated as fast and efficiently as  possible. The challenges associated with communications is the coordination of all efforts. According to Athena du Pre, lack of communication can lead to duplicated efforts, costly (and sometimes life-threatening) delays, frustration, and wasted time (Du Pre, A., 2003). During crisis events delays and wasted time can cost lives. This means that our team must keep in constant communication with other organizations to prevent overlapping efforts. Crisis communication is an ongoing process. Having a crisis communication plan is important so that more time can be spent acting and less time be spent making decisions. Examining the communication process of other crisis events can be a useful way to identify successful strategies. In 1979, the Three Mile Island reactor malfunctioned. The major form of communication used to report the event at the time were televisions and radios. Access to information was limited and local television and radio station bore the bulk of public inquiries. Organization communications had to be accomplished via telephones or face-to-face. Public communications were also limited to telephones and face-to-face. In 2005, Hurricane Katrina ravaged the city of New Orleans and left a large portion of it underwater. The major forms of communication during this event included television, radio, and the internet. With this event the internet provided quick connection and communication with multiple entities. Information could also be transferred on faste r and larger scale. However, with this particular event a large portion of the public had to go without electricity for an extended period of time. Without electricity television, radios, and the internet cannot be operated. Smartphones existed at the time, but were not as abundant. Those with smartphones and cell phones had a small window for communications after the loss of electricity. Unfortunately, these devices require recharging and are useless after so many hours. Examination of these scenarios provide proof of the importance of information access during times of crisis. In 1979, their limited resources increased the levels of public concern. In 2005, their lack of resources reduced the number of communication channels. Many people were left clueless and helpless. I believe that a good way of utilizing this knowledge is to prepare for similar situations. Publishing emergency information to the public on a wide variety of channels means more access to information, less confusion, and better coord ination of efforts. Fortunate for us, the current  scenario is not a threat to public electrical systems. However, we should be prepared for interruptions or breakdowns amongst communication channels. Each team should have contingencies in place, in case one or more resources is lost. These contingencies should include backup agencies, officials, and media sources. Teams should also have contingencies in place incase internal communications are interrupted or broken-down. Organization teams should allocate responsibilities between different members. If something happens to a team member, their responsibilities should either be divide up amongst all other team members or given to a single member. This largely depends on the size of responsibility and number of available team members. If an entire team becomes unavailable, then that teams responsibilities will be divided by the CCT and distributed amongst the remaining teams. Public contingency plans must also be addressed. In this scenario there are effectively two distinct immediate dangers. Lack of public knowledge leading to exposure, and difficulties decontaminating the public water supplies. In regards to public knowledge, it’s important that information be disseminated not only through technological communication channels, but through ground communication channels as well. Public and municipal service employees of all types should be called upon to spread the word throughout the public. The more entities that can be informed via employer to employee, the better. In regards to decontamination, the public should be fully aware that government agencies are working around the clock to solve the issue. Government entities should also offer access to alternative water supplies. During times of hurricanes, many agencies and charitable services hand out bottled water from multiple locations in their respective cities. This strategy should be utilized with the current scenario. If necessary, these services should be offered for as long as they are needed. As indicated in the previous scenarios, technology and the spread of the information go hand in hand. With the addition of the internet and digital communications, the communication process has become much more versatile. Social media often provides as a communications network for the public. Social media users can connect with other users and members of the public to share information and coordinate efforts. In today’s world many people have smartphones that are capable of internet access. These devices provide people with the ability to  access information no matter where they are. The downside to this technology is the requirement of data access. Times of crisis can overwhelm internet infrastructure, making communications slow or impossible. Short messaging services (SMS)’s are a good way of combating this effect. The benefit of SMS’s is that they operates on the mobile carrier signal rather than the connection used for mobile voice and data, allowing messages to be delivered and received on mobile devices even when the networks are too congested to make voice calls (Anonymous, 2014). Emergency alerts are also able to be broadcasted this way. Those with smartphones can be sent instant notifications; keeping them updated with current information. These systems can be used to inform a large number of people all at the same time. Compared to previous crisis scenarios, it’s possible that some technologies could be used differently. With the expansion of available communication channels, reliance on some technologies may not be as abundant as it once was. Television and radios no longer bare the sole burden of emergency broadcast communications. This can free up the efforts of local news and radio stations. Fewer public inquires to contend with, increases the overall resources of the stations. People will still call the stations for information, but the number will be significantly lower than it was in previous scenarios. In some cases television and radio may not even be utilized. Advancement in technologies has allowed the home computer to become the central h ub for information in some homes, and amongst many college students. Computers can be used for watching television and listening to radio programming. This technology increases the need for broadcasting emergency information over internet channels. Media opportunities of this management crisis include redundancy broadcast. According to a study from the University of Texas, redundancy is often necessary to overcome the noise in people’s lives because it reveals that people’s urgency perception increased after receiving three messages (Stephens, K., Barrett, A., Mahometa, M., 2013). Having the media broadcast continual information will help increase the urgency perception of the public. The more people that are made aware, the less potential there is for casualties. Social media could also be used as a way to broadcast emergency information to different groups. Sites like Facebook and Twitter, can be used to spread information. Users connected or â€Å"subscribed† to  different groups receive notifications from those groups. This allows for information to be spread quickly across a major network. Often groups will be connected to other groups. When one group learns of the emergency they can then share the information with another group, and so on. Crisis management is an urgent matter that must be handled swiftly and efficiently. Planning and communications are vital for coordinating efforts. To protect the public, organization and communication strategies and contingencies must be in place. Communication channels must be established and their advantages and challenges should be known. Applicable technologies and their utilizations should be identified. Media opportunities should also be known so that appropriate communication channels can be utilized as a means of keeping the public informed. References Anonymous. (2014). Dynmark International: SMS is most effective communication channel during emergency. (2010). Wireless News, Retrieved from http://search.proquest.com/docview/750383013?accountid=458 Du Pre, A. (2003). Communicating About Health (2nd ed.). Boston, MA: McGraw Hill HIPAA compliance. Corrections Forum, 12(1), 15-16. Retrieved from http://search.proquest.com/docview/214412170?accountid=458 Seeger, M. W. (2006). Best Practices in Crisis Communication: An Expert Panel Process. Journal of Applied Communication Research, 34(3), 232-244. doi:10.1080/00909880600769944 Stephens, K., Barrett, A., Mahometa, M. (2013). Organizational Communication in Emergencies: Using Multiple Channels and Sources to Combat Noise and Capture Attention. Human Communication Research, 39(2), 230-251. doi:10.1111/hcre.12002 Texas Department of Emergency Management (TDEM). (2014). Retrieved from http://www.txdps.state.tx.us/dem/ he Fiscal Year of 214.

Viable Cell Counting In Yeast Suspension Biology Essay

Viable Cell Counting In Yeast Suspension Biology Essay The aim of this experiment was to estimate the number of viable cells in a yeast suspension that was already provided. Estimations of the viable yeast cells were taken via two methods of plating; pour plating and spread plating, of which hot agar was used with the pour plate technique. The results that were obtained for this experiment show that overall; the spread plate method gives a higher yield of viable yeast cells compared to the total count value of 2.8 x 10^7. This experiment was conducted to estimate the number of viable cells in a yeast suspension, already provided. The definition of a viable cell, as stated in the Collins English Dictionary, 2008, p991 is capable of growth. Therefore, the definition of a viable yeast cell is a yeast cell capable of living and being able to grow. In industrial and research settings, there is a need to quantify the microbe content of microbial products. The method for doing this varies for different types of microbes. Traditionally, the first microbes to be used commercially were bacteria and yeasts. These are typically single-celled species that can be grown in natural and artificial media, and are well-suited to growth in agar gels on Petri plates. Using this method, individual cells or clumps of cells will form discrete colonies, which become visible to the naked eye as the colony grows. Counting the number of colonies provides a direct way to track the original number of discrete microbial units. A count determined this way been dubbed the number of Colony-Forming Units or CFU for short. CFUs are only applicable to single-celled microbes that can be grown on nutrient media, such as bacteria, yeasts, or spore-forming moulds. As the total count for the number of yeast cells was so vast (2.8 x 10^7) dilutions were made in order for a characteristic estimate of the total count of yeast cells to be made. Having diluted the sample enabled the human eye to count an estimate of the yeast cells. If dilutions had not been carried out, the sample of yeast cells would have been far too large and it would have been extremely time consuming and impossible to count the number of yeast cells. A haemocytometer enables for an estimate of the total number of yeast cells present. It has a known volume of chamber and area which is etched on the glass. A cell suspension is able to be above the known area. The chamber is then filled with a yeast suspension then covered with a cover slip. An average number of microbes can then be counted in the ruled area to give the number of yeast cells per cm ³. The aim of this experiment was essentially, to estimate the total number of yeast cells in a culture and to estimate the number of living (viable) yeast cells. Materials and Methods The total count of the yeast cells originally estimated by the haemocytometer was 3.8 x 10^7, however, it was later concluded that this was incorrect due to a mix up from another class. The new result for the estimated total count of the yeast cells was 2.8 x 10^7. This number was clearly too large and a series of ten fold dilutions were carried out in order to make it easier to estimate and investigate the viable yeast cells. A series of ten fold dilutions were needed as this is an important technique in identifying the viable cells. As a figure of 2.8 x 10^7 was established and it is vital that the number of colonies attained remains within the range of 30-300. So the dilution for a range of 30 300 is 1/100 (10^-2), however, it is essential that further dilutions, both above and below 1/100 are used; 1/10 (10^-1) and 1/1000 (10^-3). To make the estimation more accurate, dilutions of 10^-4, 10^-5 and 10^-6 were also used for both pour and spread plates. For full method, please refer to introduction to biology, microbiology and pharmacology practical booklet, pp 13-14. Results The results obtained for the pour plate and spread plate methods were as follow: 10^-1 10^-2 10^-3 10^-4 10^-5 10^-6 Pour plate (ml) TNTC TNTC TNTC TNTC 83 8 Spread plate (ml) TNTC TNTC TNTC 100 10 1 Key: TNTC Too numerous to count To determine the number of colony forming units (CFU) cm^-3 this calculation was used: Counts on plate x (1/dilution) x (1/volume inoculated (ml)) The calculations that were carried in order to determine the number of colony forming units (CFU) cm^-3 of the original culture for the pour plate and spread plate are shown below: Calculations for pour plate method: 83 x 1/10^-5 a 1/1 = 8.3 x 10^6 CFU, ml Calculations for spread plate method: 100 x 1/10^-4 x 1/0.1 = 1.0 x 10^7 CFU, ml The volumes inoculated for the pour and spread plate were different, the pour plate was inoculated with 1.0cm^-3 and the spread plate with 0.1micrometer. Discussion The table in the results sections shows that the values of the colonies that were counted for each of the plating techniques show good continuation, especially with the spread plate as the figures are increasing by a factor of ten each time. The figure obtained for the total count was 2.8 x 10^7, comparing this to the figure calculated for the pour plate method, 8.3 x 10^6 CFU, ml there has been a loss in the number of viable cells using this method, there has been a decrease of 1.97 x 10^7 of viable yeast cells. Comparing the total count value to the spread plate figure of 1.0 x 10^7 there was also a loss of viable yeast cells, with a loss of 1.8 x 10^7. This decrease in viable yeast cells compared to the pour plate loss is lower. The hot agar used in the pour plate technique may injure or kill sensitive cells; thus spread plates sometimes give higher counts than pour plates.(p 130, Microbiology, Seventh Edition, Joanne M. Willey et al) The above statement backs up the results of the experiment, as the spread plate technique has given a considerable higher count of viable yeast cells. Other factors that may have resulted in the smaller number of viable yeast cells in the pour plate method could have been that there is a much higher likelihood that clumps of the colonies may have formed together in portions of the plate, making it much more difficult to count. This occurs less in spread plating, as the clumps are broken up, and therefore there is a better distribution of the cells. Other factors that may have affected the results obtained for this experiment were the techniques used for the serial dilutions. With each sequential serial dilution step, there may have been transfer inaccuracies that lead to less accurate and less precise dispensing. This meant that the highest dilutions had the highest number of inaccuracies. Also, after every inoculation, the dilution must be thoroughly mixed; this was not carried during any of the dilutions, so this may have also affected the number of viable yeast cells. Finally, when doing viable counts, the higher dilution is, the more error is found in estimating the count of the original volume. For example, there were 10 colonies growing on the 10^-5 spread plate, and it was estimated that there were approximately 500000 colonies in the original suspension, but this was only an estimation to the closest hundred thousand. Likewise, with higher dilutions, such as the 10^6 on the pour plate, it was only estimated to the closest million. There were some limitations to the experiment, which may have altered the results slightly. Not having much experience in using the Gilson pipettes may have had an impact on the accuracy of the pipetting that was done during the serial dilutions.

Horizontal Communication Advantages and Disadvantages

Horizontal Communication Advantages and Disadvantages A barrier to communication as defined by Waltman A communication barrier is anything that impedes the communication process. These barriers are inevitable. While they cannot be avoided, both the sender and receiver can work to minimize them. One of the main Barriers to effective communication is the channel, shown below is a diagram that shows how effective each channel is. A communication channel as defined by Waltman (2010) Communication channels-or the media through which messages are sent-can have an influence on the success of communication In this case the channel that was used by Leigh Randell was a memo so as on the media richness graph it is listed on the bottom half of the triangle which is lower media richness. Media Richness definition as stated in Mcshane and Glinow (2003) the data-carrying capacity of a communication medium including the volume and variety of information it can transmit. By choosing to write a memo Leigh Randell has used a channel that is not as effective as a Telephone call or a Face to face talk to communicate with Tom Ballard. Therefore the impact it would have on Tom Ballard was minor. For instances if she called and explained her idea to him, she could have got an instant reply from Tom Ballard and could have got his ideas also on the strategy she was developing. So this is one of the main Barriers to effective communication. Information Overload The Next Barrier is information overload as defined in Mcshane and Glinow (2003) A condition in which the volume of information received exceeds the persons capacity to process it. As stated in the case study Leigh Randell did not get any information from Tom Ballard. When questioned about the reason why he dint reply to the memo, he answered Too busy this could be a very realistic situation in which case he has so much of work that he cant even reply to memos. This could be a problem in the organization, which therefore have a major impact on the communication process. This problem can be solved to some extent by dividing the organization employees into teams there by dividing the work load. Authority, Gender and Attitude towards the sender In this case we could say that there is a problem of authority, this could be seen by the statement of Tom Ballard Besides, I dont report to her. This clearly shows that Tom Ballard is a person who does not consider ideas from people in the same level or lower levels on his job/field. Because Leigh Randell was in the same level in the organization hierarchy (shown below) Tom Ballard did not want to reply. Organization Layout Model Exhibit: Omega Airlines, Atlanta Organization Hierarchy Also we could see that gender also has an issue in this case. This is clearly seen in the statements by Tom Ballard To busy, Her question was out of sight. There was no time for me to answer this sort of request. Ive got a job to do. This shows that even though Leigh Randell was more senior in terms of experience than Tom Ballard he dint consider it and decided to ignore her memos. This also can be related to the attitude towards the sender which is another barrier that causes a lot of problems in effective communication. No, it dint look like that to me, I also had ideas on how to improve the system This shows that Tom Ballard (male) thinks his ideas are more superior to Leigh Randell (female). This is unfair to Leigh Randell who is going out of the way to help the organization. Horizontal Communication effectiveness Horizontal communication is basically when 2 employees in the same level of the organization hierarchy communicate. And in this case the effectiveness of the communication was very low. Leigh Randell used the communication method show in red three times and had no reply from Tom Ballard. Then she used the communication method (Bottom Up) which is displayed in Green and contacted Alan Brock, which was effective. We can come to an assumption that horizontal communication is not effective because the person is not compelled to reply, unlike top down communication. We can come to a clear conclusion that because of the week horizontal communication the company Organization Communication Model Exhibit: Omega Airlines, Atlanta Communication graph 2. Is anyone wrong in this situation? By what other means could Randell have requested the information from Tom Ballard? What do you think of Tom Ballards reaction? Why? a) After analyzing the case study the wrongs are as follows, Leigh Randell and Tom Ballard did wrong in various ways. First of all focusing on Leigh Randells wrong, she used a channel not suitable for inquires like that. She should have used a channel that has higher media richness such as a telephone call or a face to face discussion. Also she should have switched the communication channel after she sent the memo twice and had no reply she should have got some idea that the channel is defective. But she continued to send memos to Tom Ballard. This is wrong on her part for choosing a bad channel and kept on using the same channel after not getting any results. Next taking into consideration the wrong committed by Tom Ballard, he was wrong to read the memos and not reply to them. By not providing feedback to Leigh Randell, she was not able to continue her work, which could have beneficial to the organization. Also having own idea on the same topic does not mean others have no right to express their views. He was being very self-centred about his thinking method. This could be clearly seen from his comments at the meeting, To busy, Her question was out of sight. There was no time for me to answer this sort of request. Ive got a job to do. So finally we could say both parties committed wrong but more weighted on Tom Ballard for his ignorance and his attitudes, and for Leigh Randell she is been more traditional way of acquiring information. b) Leigh Randell had many other ways and means to contact Tom Ballard such as, Face to face meeting Video Conference Telephone call Using these modes of communication would have had a much more fruitful outcome, because media richness is high in these modes. In other words the variety and amount of information that could have been transmitted is higher. These are the recommended channels to achieve the best results. The more modern modes will be discussed in the 3rd question under recommendations but few of them are as follows. 3G Calls/ webcam Instant Messaging Google Wave Explanations will be in the recommendation part. C) Tom Ballards characters as depicted by his comments are very self cantered and arrogant in a way. This is in one way a disadvantage for him and his co-workers. This can be seen by his statements given below To busy, Her question was out of sight. There was no time for me to answer this sort of request. Ive got a job to do, Besides, I dont report to her NO, It dint look like that to me. You know i.ve also had ideas on how to improve the system for quite some time. Anyway she is going about it all wrong The behaviour shows us a basic masculine instinct of trying to show who has more power, in other words dominance. He felt threaten by her because she was trying to do his job. By Tom Ballards action it is possible to say that he is not a team player and like to work alone. This is totally unacceptable in an organization that needs allot of coordination. Also by his comment You know Ive also had ideas on how to improve the system for quite some time. This shows that he does not want express his ideas on how to improve the company but keeps them to himself. Also showing us, he does not feel part of the company. 3. a) While communicating vertically up or down the organization does not present a major problem, why is horizontal communication more difficult to attain? b) Give your recommendations to improve communication in this organization To explain this the following model is down below Organization communication model Horizontal Communication Exhibit: Omega Airlines, Atlanta Communication graph The arrow displayed in green represents horizontal communication, the green box displays the horizontal communication happening in the organization. The reason it is not effective can be that Problems In Horizontal Communication Solutions Both have equal authority/ same level of power Organization trips Mismatch of Ideas between peers in same level Get-togethers Competitiveness in the organization Organizational Parties Attitudes towards other peers Team building workshops These are problem most organizations faces and have taken steps to increase the effectiveness of horizontal communication. The importance of horizontal communication is explained by Juneja, H (2009) horizontal communication in an organization which is also very important. It is essential that people working at same level should have effective communication amongst them so that there is co-ordination between them. Workers are more likely to be more serious when it comes to communication with their superiors In this case Kent Davis summoned a hastily meeting to solve the issue that had happen in this horizontal communication, showing us that the organization is taking its communication issues seriously. Especially for an Airline which requires allot of co-ordination. For an Airlines or any organization to compete in this modern world they require the best state of the art technology in communication available. This is because communication is a key in achieving any objective. Omega Airlines needs to improve its communication technology to overcome future problems. A quote from an article by Baker, K.A. (2002) , new communication technologies can enable almost every aspect of organizational management and effectiveness, including change management, knowledge management, participative management, innovation, and organizational partnerships and alliances Server/ Database Creation My recommendation firstly to build a Server or Database where only organization employees can access and store all data that can be used by other employees. This will not only speed up the information distribution and communication process but also organize the data in one place so all records can be viewed 24/7 365 days. Source: Google images 2010 Video Conferencing/ webcam/ 3G calls This is a new way that modern companies contact employees, because the media richness is higher in a video call than a normal telephone call. And it is proven than visual communication has a bigger impact or stays in the brain/ memory much longer. Source: Cisco Official Website 2010 Instant Messaging These are Messaging programs that are very user friendly and do not take long to reply. For example when sending an email you have to get the address and then after sending you have to wait for a reply. But with instant messaging software its not necessary, once the person comes to the organization he can login and any message will be sent and replied to instantly. Also the best thing its all free. Source: Google images 2010 Google Wave Source: Google wave website 2010 Google wave is a new technology that is revolutionizing the organization communication process. The description as given in Google (2010) Google Wave is an online tool for real-time communication and collaboration, A wave can be both a conversation and a document where people can discuss and work together using richly formatted text, photos, videos, maps, and more. In other words this plays the roles of an instant messenger, video conference software also it has the features of an email message. This is the future. As a conclusion, by implementing these technologies all aspects of the organization will be improved drastically, especially in organization such as this where communication is KEY. Having technology is one thing but for people to adapt is the difficult part. But all the technology and software that was said here are very user friendly easy to use and easy to update. There by not only will make the organization a better more efficient organization but also an up to date one. And will give an assurance that incident like this between Leigh Randell and Tom Ballard not happen again.